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C7642 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

FITC conjugate, Type IV, lyophilized powder

Synonym: ConA

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Concanavalin A (Con A), Fluorescent Detecton on SDS-PAGE Gels, Glycan Recognizing Proteins,
type   Type IV
form   lyophilized powder
composition   Protein, 5-15% biuret
extent of labeling   3-6 mol FITC per mol protein
impurities   Carbohydrate, essentially free
conjugate   FITC conjugate
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

For the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

2, 10 mg in glass bottle

Package size based on protein content

Physical form

Lyophilized powder containing phosphate buffer salts and NaCl

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Fluorescent Detection on SDS-PAGE Gels

Identify glycoproteins with superior selectivity. Allows fluorescent detection of glycoproteins directly in PAGE gels without blotting or use of antibodies.
Glycobiology Analysis Manual, 2nd Edition
Keywords: Applications, PAGE

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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