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C7706 Sigma

Monoclonal Anti-VSV Glycoprotein−Cy3 antibody produced in mouse

clone P5D4, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-VSV Glycoprotein

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, Molecular Biology, Primary Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   P5D4, monoclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct
isotype   IgG1
conjugate   CY3 conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy is a registered trademark of GE Healthcare

Cy3 is a trademark of GE Healthcare

Specificity

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

A dilution of 1:1000 of Monoclonal Anti-VSV Glycoprotein-Cy3® antibody produced in mouse was used to detect VSV glycoprotein in pig sperms by immunofluorescence.

Biochem/physiol Actions

The vesicular stomatitis virus (VSV) glycoprotein facilitates the entry of HIV into the CD4 T cells through endocytotic pathway. The coupling of Cy3 to Anti-VSV Glycoprotein antibody allows for the visualization of protein by fluorescent microscopy.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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