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C7729 Sigma

Anti-Caspase 9 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Parkinson’s Disease Research,
species reactivity   human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart
  immunoprecipitation: 10 μg using HeLa mitochondrial RIPA lysate (500 μg)
  microarray: suitable
  western blot: 1:300 using whole extract of human Jurkat cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 35-47 kDa (two bands)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP9(842)
rat ... Casp9(58918)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde.

General description

Caspases are cysteine proteases that mainly regulate apoptosis signaling. Caspase 9 is activated by the apoptosome complex and initiates apoptosis via the mitochondrial route . Anti-Caspase 9 specifically recognizes the proform and the active cleaved form of human caspase 9, but cross-reactivity has also been observed in rats. Staining of caspase 9 by immunoblotting is specifically inhibited with the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Caspase 9 antibody is suitable for use in immunoprecipitation (10 μg using 500 μg HeLa mitochondrial RIPA lysate), microarray and western blot (1:300 using whole extract of human Jurkat cells). The antibody may also be used in immunohistochemistry (in formalin-fixed, paraffin-embedded sections at 1:40 dilution, using microwave-treated tissue sections of human and rat heart). Additionally, the antibody product is useful for immunoblotting and immunoprecipitation (approx. 47 kDa and 35 kDa) assays. An additional band of higher molecular weight (approx. 70 kDa) may also be detected in some cell line extracts.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Parkinson's Disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurod...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 16–20
Keywords: Alzheimer Disease, Apoptosis, Chromatin immunoprecipitation, Clinical, Degradations, Dopamine agents, Gene expression, Parkinson Disease, Polymorphisms

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Peer-Reviewed Papers
15

References

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