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C7736 Sigma

Anti-Centrin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:2,000 using 293-T exp.Hu.Centrin-1LY117608 or 293-T exp.Hu.Centrin1-FLAG LY401316
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 21 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CETN1(1068)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human centrin 1 (amino acids 157-172). The sequence is highly conserved in centrin 1 of many species, e.g. mouse, Drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.

General description

The centrosome, the major microtubule-organizing center (MTOC) in the animal cell, is a key determinant in the organization of the cytoplasm, in the determination of cell polarity, and in the establishment of mitotic spindle poles during the cell cycle. Centrin is a member of the calcium binding E-F hand protein superfamily present in the centrosome pericentriolar matrix.
Three separate centrin genes have been identified in human cells: centrin 1, 2, and 3. Centrin 1 shows strong amino acid homology to centrin 2 (85%), but lower homology to centrin 3 (54%) and CDC31 (50%) from the yeast S. cerevisiae. Centrin has a fundamental role in the MTOC structure and function. Phenotypic analysis of mutants in Chlamydomonas caltractin and the yeast CDC31 genes have indicated that the protein is required for proper duplication and segregation of the MTOC in the respective cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Centrin antibody is suitable for immunoblotting: a minimum working dilution of 1:2,000 is recommended using 293-T exp.Hu.Centrin-1LY117608 or 293-T exp.Hu.Centrin1-FLAG LY401316.

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