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C7805 Sigma

Monoclonal Anti-Collagen, Type III antibody produced in mouse

clone FH-7A, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Mesenchymal Stem Cells,
species reactivity   human, rat
application(s)   dot blot: suitable
  immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
  indirect ELISA: suitable
  western blot: suitable using a denatured-reduced preparation
clone   FH-7A, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 70 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL3A1(1281)
rat ... Col3a1(84032)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human collagen type III

General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X. This product has also shown reactivity for rat and human collagen type III.

Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

Specificity

Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X.

Application

Monoclonal Anti-Collagen, Type III may be used for ELISA, dot-blot, immunoblotting (approx. 70 kD in denatured-reduced preparations) and in immunohistochemistry (frozen sections). Reactivity has been observed with human and rat collagen type III.
Antibodies that react specifically with collagen type III are useful for the study of specific differential tissue expression and the immunolocalization of collagen type III in normal and neoplastic tissue.

Monoclonal anti-collagen type III antibody can be used in immunofluorescence and western blot.

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