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C7831 Sigma

Anti-CD28 antibody, Mouse monoclonal

clone CD28.2, purified from hybridoma cell culture

Synonym: Monoclonal Anti-CD28

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   rhesus monkey, human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
  immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
clone   CD28.2, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 44 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD28(940)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human CD28 transfected murine T cell hybridoma.

General description

CD28 (Cluster of differentiation 28) is a glycosylated membrane protein. The protein exists as a homodimer.

Antigen Background

CD28 is expressed on thymocytes, peripheral lineage cell subsets, activated B cells and plasma cells. The antigen is involved in adhesion between T and B lymphocytes through the B7/BB-1 ligand expressed on activated B cells. CD28 co-stimulates T-cells activated via the CD3 T-cell receptor and CD2 pathways and induces their proliferation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD28 (44 kDa) human cell surface disulfide-linked, homodimeric glycoprotein. The antibody reacts with human CD28-transfected murine T-cell hybridoma, PHA-activated blasts and IL-6 dependent plasmacytoma lines. It cross reacts with rhesus monkey PBL. The antibody blocks CD28/B7 (CD80) mediated cell-cell adhesion. It binds to the CDR domain in a CDR1 dependent manner. It enhances CD3 monoclonal antibody-induced T-cell proliferation. May be used in adhesion and co-stimulation studies.
5th Workshop: code no. CD28.05.

Application

Monoclonal Anti-CD28 antibody produced in mouse is suitable for the following applications:
• Flow cytometry at a dilution of 5μL using 1 × 106 cells
• Immunocytochemistry
• Immunohistochemistry (frozen sections)
The antibody has been used for development of an assay to identify small molecules antagonists of CD28/CD86 binding.

Biochem/physiol Actions

CD28 (Cluster of differentiation 28) is a protein expressed on T-cells that provide co-stimulatory signals required for T-cell activation, survival and differentiation into effector T cells. CD28 is also expressed on multiple myeloma (MM) cells and is a key mediator of MM survival and apoptotic resistance. CD28 pro-survival signaling is dependent upon downstream activation of phosphatidylinositol 3-kinase (PI3K)/AKT (RAC-alpha serine/threonine-protein kinase), inactivation of the transcription factor FoxO3a and decreased expression of the pro-apoptotic molecule BIM. CD28 plays an important role as a mediator of MM survival during stress and can be targeted to overcome chemotherapy resistance. Inflammatory CD28- T-cells are found to be accumulated in livers of patients with primary sclerosing cholangitis (PSC) and are localized around bile ducts. Autoantibodies against CD28 are found in patients with autoimmune and atopic diseases.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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