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C7849 Sigma

Anti-pro-Caspase 8 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:1,000 using a whole cell extract from the human Burkitt lymphoma Raji cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen (caspase 8a) mol wt 55 kDa
  antigen (caspase 8b) mol wt 54 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP8(841)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal region (amino acids 2-20) of human caspase 8 N-terminal prodomain, with C-terminal added lysine, conjugated to KLH.

General description

Caspase belongs to the family of cysteine proteases and plays crucial roles in extrinsic apoptosis, inflammation, and cellular differentiation. There are at least 14 different caspases with different substrate specificity. Caspase 8 was first identified in humans and at least eight different caspase 8 isoforms have been identified at mRNA level. The predominant caspase 8 isoforms expressed in cells. Caspase 8a/b (479 and 464 a.a.), are formed as inactive 55kDa and 54kDa precursors, respectively. The N-terminal region of caspase 8 contains two death effector domains (DEDs) which facilitate the interaction with the adaptor protein FADD/MORT-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

The sequence used as the immunogen is identical in human caspase 8 isoforms (MACHα1, MACHα2, MACHβ1, MACHβ3, MACHβ4) and has limited homology (60%) to mouse caspase 8. By immunoblotting, the antibody reacts with caspase 8a and caspase 8b.

Application

Anti-pro-Caspase 8 antibody was used for immunoblotting of protein in a study of a novel short isoform of caspase-8 (caspase-8s), which encodes the first Death Effector Domain (DED) and part of the second DED, missing the C-terminal caspase domain. It is also suitable for microarray and western blotting analysis at a dilution of 1:1,000 by using a whole cell extract from the human Burkitt lymphoma Raji cell line.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

The activation of anti-pro-Caspase 8 antibody needs oligomerization-induced processing. It subsequently removes its death domain and cleavage into active 20kD and 10kD subunits which further released in the cytosol. These subunits form a proteolytically active heterodimer which are capable of cleaving other downstream caspase family members.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
15

References

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