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C7852 Sigma

Anti-CAD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Caspase Activated DNase I, Anti-DFF40, Anti-DNA Fragmented Factor 40

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to DNA Fragmentation,
species reactivity   mouse
application(s)   western blot: 1 μg/mL using mouse lung tissue lysate
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 40 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Dffb(13368)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 205-222 of mouse CAD (cell death-inducing DFF-like effector A).

General description

Caspase Activated DNase is a heterodimeric protein composed of ICAD and CAD (in human known as DFF45 and DFF40, respectively) two subunits, which triggers DNA fragmentation during apoptosis.

Physical form

Solution in phosphate buffered saline, containing 0.02% sodium azide.

Application

Anti-CAD antibody was used for western blotting in a study of simultaneous downregulation of uPAR (urokinase-type plasminogen activator receptor] and Cathepsin B induces the up-regulation of pro-apoptotic genes.

Biochem/physiol Actions

In inactive state DFF exists as a cytoplasmic protein. After activated by apoptotic signals DFF45 functions as a chaperone, mediating the correct folding of DFF40, as well as an inhibitor of DFF40. In response to apoptotic signals, DFF45 is cleaved by caspase-3 at two sites; which results releasing of active nuclease, DFF40. Furthermore, DFF40 oligomerize to form a large, functional complex which further breaks down DNA by introducing double-strand breaks. However, DFF40 interact directly with histone H1 which may stimulate its activity.

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