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C7855 Sigma

Monoclonal Anti-Cdh1 antibody produced in mouse

~2 mg/mL, clone DCS-266, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cell Cycle Regulators,
species reactivity   human
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 2-4 μg/mL using a HeLa cell nuclear extract
clone   DCS-266, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FZR1(51343)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human Cdh1.

General description

Cdh1 homophilic major cell adhesion molecule in epithelial cells belongs to cadherin superfamily. It mediates Ca2+ dependent homophilic interactions, as the major adhesion receptor in adherens junctions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Application

Monoclonal Anti-Cdh1 antibody was used for immunoblotting of lysates in a study of Identification of Novel Human Cdt1-binding Proteins. It is suitable for western blotting with 2-4 μg/mL by using a HeLa cell nuclear extract. It is also suitable for immunoprecipitation and microarray.

Biochem/physiol Actions

Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. In eukaryotes, regulation of cell cycle progression depends on the expression of cyclins proteins. Degradation of mitotic cyclins follows ubiquitin-dependent pathways. The anaphase-promoting complex/cyclosome (APC/C) plays a vital role in progression. Initially at anaphase the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but later it require Cdh1 to maintain its active state till the next S phase. Another cyclin A/cdk2 mediated phosphorylation of Cdh1 may also prevents the activatory assembly of Cdh1 with APC, thus creating an environment permissive for accumulation of APC targets.

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