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C7898 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

ferritin conjugate, Type IV, buffered aqueous glycerol solution

Synonym: ConA

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Properties

Related Categories Concanavalin A (Con A), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
type   Type IV
form   buffered aqueous glycerol solution
concentration   5-10 mg/mL protein (Biuret)
extent of labeling   ~1 mol ferritin per mol lectin
impurities   carbohydrate, essentially free
conjugate   ferritin conjugate
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

Package size based on protein content

Physical form

Solution in 50% glycerol containing 0.3 M NaCl, 0.01 M sodium phosphate, 0.06 M CaCl2, 0.06 mM MnCl2 and 0.02% sodium azide as preservative

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles
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