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C7901 Sigma

Chelex® 100 sodium form

50-100 mesh (dry)

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Properties

Related Categories Affinity Chromatography, Affinity Resins, Chelating Resins, Core Bioreagents, Molecular Biology,
matrix   1% cross-linked polystyrene
  cross-linked polystyrene beads
matrix active group   iminodiacetic acid (sodium form)
  paired iminodiacetate groups
matrix attachment   through methyl group to aromatic rings
particle size   50-100 mesh (dry)
pH range   4 - 14
capacity   ≥0.3 meq/mL binding capacity
  0.6 meq/g binding capacity (heavy metal ions)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

25, 50, 100, 500 g in poly bottle

Application

Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What solvent should I use to swell the Product C7901, Chelex 100 at the beginning?
We recommend using a good grade of water (deionized or distilled).
My Chelex 100 (Product C7901) in my column dried out.  Can I still use it?
The resin may be rehydrated and used, as long as it remains white.  If the color of the re-swollen resin is yellow, do not use it.
I want to use Product C7901, Chelex 100, to make a metal-free solution.  However, the water wash from the Chelex resin is very basic.  How can I fix this?
A suspension of Chelex 100 (sodium form) in water typically has a pH of 10-11. This pH can be slowly lowered by extended water washing, or by using a buffer.  The sodium form at pH 6.3 can be obtained by rinsing with at least 4 bed volumes of 0.5 N sodium acetate buffer, followed by 5 bed volumes of water. This is a more common method for making metal-free distilled water.
What is the particle size of the resin in Product C7901, Chelex 100?
The particle size for this resin is 45-165 microns. The mean particle size is 90 microns.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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