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C7911 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type III-ASCL, saline suspension

Synonym: Con A

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Properties

Related Categories Concanavalin A (Con A), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
type   Type III-ASCL
form   saline suspension
extent of labeling   ~10 mg per mL
matrix   4% cross-linked beaded agarose
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ~10 mg/mL binding capacity (peroxidase (P 8375))(pH 6.0)
  ~6 mg/mL binding capacity (yeast mannan)(pH 7.2)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Quantity

One mL suspension yields approx. 0.5 ml gel.

Physical form

Suspension in 1.0 M NaCl containing 1 mM CaCl2, 1 mM MgCl2, 1 mM MnCl2 and 0.02% thimerosal

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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