• USA Home
  • C7923 - Monoclonal Anti-CD44 antibody produced in mouse

C7923 Sigma

Monoclonal Anti-CD44 antibody produced in mouse

clone A3D8, purified immunoglobulin, buffered aqueous solution




circulating malignant human Sezary T cells.

General description

CD44 is a glycosylated cartilage-linked protein. The protein exists in a variety of forms with different molecular structures, ranging from 85 kDa to 250 kDa. It is a multistructural and multifunctional cell surface molecule and involved in various biological processes such as cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. Although all the activities are related to normal cell, but it has also some pathologic activities of cancer cells.

Antigen Background

The CD44 antigen has been studied under the following names: Pgp-1, Hermes antigen, ECM-III, H-CAM and HUTCH-1. It functions as a homing receptor. CD44 antigen is the cellular receptor for hyaluronic acid. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Recognizes the CD44 (80-95 kDa) human cell surface glycoprotein on B and T lymphocytes, monocytes, granulocytes and red blood cells. The epitope recognized by this antibody is sensitive to bromelain and other proteolytic enzymes. It is detectable in routine formalin-fixed, paraffin-embedded tissue.
5th Workshop: code no. BP406, S320


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-CD44 antibody was used as a hyaluronic acid receptor for indirect immunoperoxidase technique in a study to evaluate the relationship of lymphoid cells and macrophages with vasculature and stromal components.
Anti CD44 monoclonal antibody was used for:
• immunostaining of nonpapillary carcinoma fine-needle aspiration (FNA) or surgical excision specimen.
• flow cytometry analysis to assess breast CSCs in human MCF-7/Dox breast cancer cells.
• staining of CD44 in immunohistochemistry analysis.
• incubation of osteoclasts (OCs) in a study to analyze the molecular composition and F-actin organization of the OC podosome belts.
• It is also suitable for western blot at a dilution of 1:500-1:2,000

Biochem/physiol Actions

The CD44 antigen is expressed on a variety of cell types including peripheral blood leukocytes (B and T lymphocytes, monocytes, granuloctes) and red cells. It is also weakly expressed on platelets. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells. The epitope recognized by this clone is sensitive to formalin fixation and paraffin embedding.

Price and Availability

Antibody Explorer

Explore with Confidence

All labs need water
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot


Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?