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  • C7974 - Monoclonal Anti-Collagen, Type X antibody produced in mouse

C7974 Sigma

Monoclonal Anti-Collagen, Type X antibody produced in mouse

clone COL-10, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   human, deer, pig
application(s)   dot blot: suitable
  immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells
  western blot: suitable
clone   COL-10, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 60 kDa (in denatured-reduced preparations)
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL10A1(1300)
biological source   mouse
conjugate   unconjugated



purified pig collagen type X.

General description

Collagens are extracellular glycoproteins made up of three polypeptides that intermingle to form a triple helix. Type X collagen is a homotrimer of 59 kD α1(X) chains found in fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs, whereas in adults it is also present in thyroid cartilage. It acts as a scaffold to avoid local collapse during endochondral ossification and also has a role in cartilage mineralization. Monoclonal anti-collagen, type X antibody can be used in histological and immunohistochemical evaluation of cell cultures. It is also useful in study of specific differential tissue expression. Mouse anti-collagen, type X antibody reacts specifically with native and denatured collagen type X but not with collagen types I, II, III, V, IX and XI. The product has also shown reactivity for collagen X of porcine, deer and human.


Monoclonal anti-collagen, type X antibody can be used in ELISA, immunoblotting and dot blot. It is also useful for the immunolocalization of collagen type X.

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RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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