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C8034 Sigma

Anti-Clathrin antibody produced in goat

whole antiserum

Properties

Related Categories Alphabetical Index, Antibodies, CI-CN, Primary Antibodies
species reactivity   human
application(s)   indirect immunofluorescence: 1:20 using human foreskin fibroblasts
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CLTC(1213)
biological source   goat
conjugate   unconjugated

Description

Immunogen

clathrin from bovine brain coated vesicles

General description

Clathrin is consists of three heavy chains, along with three light chains. The weight of heavy chains varies depending on the source of the clathrin. It forms a three-legged structure termed a triskelion with three clathrin heavy chains (molecular weight 180,000 daltons per chain) and three non-covalently bound light chains having molecular weights of 30,000-40,000 daltons per chain. Although the term clathrin was originally used to designate the heavy chain component of triskelions, clathrin is now used to refer to the entire triskelion protein molecule.

Specificity

The product specifically labels the heavy chain band of clathrin by immunoblotting.

Application

Anti-Clathrin antibody was used:
• for incubating trophozites (2.105) in a study to determine the role of clathrin in endocytic process of fluid-phase pathway.
• in indirect immunofluorescence staining.
• for immunocytohistochemistry study of human platelets to study the colocalization of adhesive proteins with clathrin in human blood platelets.

Biochem/physiol Actions

Clathrin is the main constituent of the polygonal network that forms the layer of coated vesicles and coated pits. It is widely assumed that coated vesicles mediate the selective transfer of molecules and membrane components between specific membranous organelles within cells. It is a type of protein which plays an important function within the cell, including various types of intracellular transport processes.

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