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  • C8035 - Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse

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C8035 Sigma

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse

clone CS-56, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   chicken, rabbit, human, pig, rat, bovine, mouse
application(s)   indirect immunofluorescence: 1:200 using bovine mammary gland epithelial (BMGE) cells
  microarray: suitable
clone   CS-56, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACAN(176)
mouse ... Acan(11595)
rat ... Acan(58968)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

ventral membranes of chicken gizzard fibroblasts

Specificity

The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.

Application

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse was used:
• in immunohistochemistry as primary antibody to study of spinal cords and brain cryosections.
• in ELISA and western blot for purification of soluble forms of myelin-associated glycoprotein (MAG) and neural cell adhesion molecule (N-CAM).
• in flow cytometry studies.
• as primary antisera in a study of TGF-β regulation.
Monoclonal Anti-Chondroitin Sulfate can also be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts. It is also suitable for microarray and indirect immunofluorescence at the dilution of 1:200 by using bovine mammary gland epithelial (BMGE) cells.

Biochem/physiol Actions

Chondroitin sulfate is involved in tumor invasion and also plays a vital role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. In Chondroitin sulfate proteoglycan (CSPG), proteoglycan part is involved in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis.

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