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C8052 Sigma

Cholera Toxin from Vibrio cholerae

~95% (SDS-PAGE), lyophilized powder

Synonym: CTX, Cholera enterotoxin, Cholergen

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Properties

Related Categories Bacteria, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, C-CH, Cell Biology,
Quality Level   PREMIUM
assay   ~95% (SDS-PAGE)
form   lyophilized powder
composition   Protein, ~5% Lowry-TCA
solubility   H2O: 10 mg/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

Package size based on protein content.

Reconstitution

When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA

Application

Cholera Toxin from Vibrio cholerae has been used as a positive control in cAMP (cyclic AMP) assay for enterotoxins. It has been added as a supplement in cell culture media of primary tumors and epithelial cells.

Biochem/physiol Actions

Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3462 6.1 / PGII
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the molecular weight Product C8052, Cholera enterotoxin?
Although the molecular weight is not part of our specifications for this product, it has been reported to be 84 kDa in Biochimica Biophysica Acta, 492, 303 (1977).
Is Product C8052, Cholera enterotoxin, sterile?
It is not necessarily sterile, but the solution can be filter sterilized using a 0.2 micron pore size filter.
What is the solubility and solution stability of Product C8052, Cholera enterotoxin?
Cholera toxin is soluble in water at a concentration of 10 mg/mL. Solutions are reported to be stable for 1 year when stored at 2-8 °C, and will lose biological activity after prolonged exposure to pH below 6 or above 8, according to Mekalanos, J.J., et al., Meth. Enzymology, 165, 169-175 (1988).
How is the activity of Product C8052, Cholera enterotoxin, expressed?
The activity is expressed in terms of units/mg of protein and not as units/mL.
Is Product C8052, Cholera enterotoxin, cell culture tested?
This product is not cell culture tested.
What is the shelf life of Product C8052, Cholera enterotoxin?
Each lot of C8052 is given a retest date of three years from the QC acceptance date.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Discover Bioactive Small Molecules for Cyclic Nucleotides

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Peer-Reviewed Papers
15

References

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