Cholera Toxin from Vibrio cholerae
~95% (SDS-PAGE), lyophilized powder, 1 × 105-1 × 106 units/mg protein
DOWNLOAD MSDS (PDF)Synonym: Cholera enterotoxin, Cholergen
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CAS Number 9012-63-9
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MDL number MFCD00130776
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling and Neuroscience, |
| Quality Level | PREMIUM |
| assay | ~95% (SDS-PAGE) |
| form | lyophilized powder |
| composition | Protein, ~5% Lowry-TCA |
| solubility | H2O: soluble10 mg/mL |
| storage temp. | 2-8°C |
Description
Frequently Asked Questions
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Packaging
Package size based on protein content.
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthi
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
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