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C8093 Sigma

Monoclonal Anti-Connexin-43 antibody produced in mouse

clone CXN-6, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Stem Cell Biology,
species reactivity   bovine, mouse, human, hamster, rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:8,000-1:16.000 using mouse whole brain extract
clone   CXN-6, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 43 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GJA1(2697), GJA1P1(2698)
mouse ... Gja1(14609)
rat ... Gja1(24392)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic connexin-43 peptide (362-381).

General description

The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes. Gap junction protein levels change in response to disruption of tissue architecture. For instance, an increased expression of Cx43 was found in early stages of atherosclerosis.
Changes in the levels or types of connexin expressed in a given cell type have been found to correlate with tumor progression and metastasis. However, glioma cells transfected with the oncogene neu (c-erb-B2) have been shown to exhibit a major reduction in intercellular communication with no decrease in overall expression of Cx43.

Specificity

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

Application

Monoclonal Anti-Connexin-43 antibody may be used for the localization of connexin-43 using various immunochemical assays such as ELISA, immunocytochemistry, immunoblotting (43 kDa, and additional weaker bands), and immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues).

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete. The expansion of the carcinogenesi...
Vicki Caligur
BioFiles 2008, 3.5, 18.
Keywords: Acetylations, Adhesion, Alkylations, Apoptosis, Biofiles, Cancer, Carcinogens, Cell division, Cell proliferation, Cell signaling, DNA microarrays, Drug discovery, Environmental, Epigenetics, Events, Gene expression, Genetic, Hormones, Metabolism, Methylations, Microarray Analysis, Mutagens, PAGE, Recombination, Reductions, Sequences, Transcription, Type, transformation

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Peer-Reviewed Papers
15

References

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