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C8104 Sigma

Monoclonal Anti-Ceramide antibody produced in mouse

clone MID 15B4, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Lipids in Cell Signaling, CE-CH,
biological source   mouse
antibody form   purified immunoglobulin
clone   MID 15B4, monoclonal
form   buffered aqueous solution
application(s)   immunohistochemistry (frozen sections): suitable
  indirect ELISA: 1:10
isotype   IgM
conjugate   unconjugated
shipped in   wet ice
storage temp.   2-8°C

Description

Analysis Note

The antibody was characterized on ceramide covalently bound to myoglobin.

Immunogen

ceramide conjugated to bovine serum albumin.

Preparation Note

Purified from ascites fluid by gel filtration using sephacryl S-300 resin.

Specificity

Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.

Physical form

Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.

Application

Mouse monoclonal clone MID 15B4 anti-Ceramide antibody is used to tag ceramide for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.

General description

Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is generated by hydrolysis of membrane sphingomyelin by a unique magnesium independent, neutral, cytosolic sphingomyelinase. Ceramide is generated in response to cellular stimulation by hormones, cytokines and antigens. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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