|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,|
|recombinant||expressed in Pichia pastoris|
|activity||≥40 units/mg protein|
|shipped in||dry ice|
|Gene Information||human ... CMA1(1215)|
Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.
Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.
One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.
Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6
Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.
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sufficient for 150 assays (100 μL), sufficient for 30 assays (1 mL)
lyophilized powder, ≥50 units/mg protein (Bradford)
buffered aqueous solution, ≥5 units/mg protein
Certificate of Analysis
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
P082 The Effects of Saponins in Flower Buds of Panax Notoginseng on Changes of TGF-β/Smads Signaling Transduction Pathway in Myocardium of Human Chymase Gene Transgenic Mouse Zhou, D., et al. Int. J. Cardiol. 147, s32, (2011)
PPAR-alpha activation as a preconditioning-like intervention in rats in vivo confers myocardial protection against acute ischaemia-reperfusion injury: involvement of PI3K-Akt. Ravingerová T, Carnická S, Nemčeková M, et al. Can. J. Physiol. Pharmacol. 90(8), 1135-44, (2012)
Computational studies of novel chymase inhibitors against cardiovascular and allergic diseases: mechanism and inhibition. Arooj M, Thangapandian S, John S, et al. Chem. Biol. Drug Des. 80(6), 862-75, (2012)
Spontaneous remodeling of HDL particles at acidic pH enhances their capacity to induce cholesterol efflux from human macrophage foam cells. Nguyen SD, Öörni K, Lee-Rueckert M, et al. J. Lipid Res. 53(10), 2115-25, (2012)
Inhibition of p38 mitogen-activated protein kinase phosphorylation decrease tert-butyl hydroperoxide-induced apoptosis in human trabecular meshwork cells. Yang Y, Liu X, Huang J, et al. Mol. Vis. 18, 2127-36, (2012)
Trichinella spiralis secreted enzymes regulate nucleotide-induced mast cell activation and release of mouse mast cell protease 1. Afferson HC, Eleftheriou E, Selkirk ME, et al. Infect. Immun. 80(11), 3761-7, (2012)
Extended cleavage specificity of the mast cell chymase from the crab-eating macaque (Macaca fascicularis): an interesting animal model for the analysis of the function of the human mast cell chymase. Thorpe M, Yu J, Boinapally V, et al. Int. Immunol. 24(12), 771-82, (2012)
Heparan sulfate 6-O-sulfotransferase isoform-dependent regulatory effects of heparin on the activities of various proteases in mast cells and the biosynthesis of 6-O-sulfated heparin. Anower-E-Khuda MF, Habuchi H, Nagai N, et al. J. Biol. Chem. 288(6), 3705-17, (2013)
Chymase-mediated intestinal epithelial permeability is regulated by a protease-activating receptor/matrix metalloproteinase-2-dependent mechanism. Groschwitz KR, Wu D, Osterfeld H, et al. Am. J. Physiol. Gastrointest. Liver Physiol. 304(5), G479-89, (2013)
Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitors. Powers JC, Tanaka T, Harper JW, et al. Biochemistry 24(8), 2048-58, (1985)
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