|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,|
|recombinant||expressed in Pichia pastoris|
|activity||≥40 units/mg protein|
|shipped in||dry ice|
|Gene Information||human ... CMA1(1215)|
Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.
Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.
One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.
Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6
Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.
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sufficient for 150 assays (100 μL), sufficient for 30 assays (1 mL)
lyophilized powder, ≥50 units/mg protein (Bradford)
buffered aqueous solution, ≥5 units/mg protein
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|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
P082 The Effects of Saponins in Flower Buds of Panax Notoginseng on Changes of TGF-β/Smads Signaling Transduction Pathway in Myocardium of Human Chymase Gene Transgenic Mouse Zhou, D., et al. Int. J. Cardiol. 147, s32, (2011)
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|CS1140||Chymase Activity Assay Kit, sufficient for 150 assays (100 μL), sufficient for 30 assays (1 mL)|
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