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C8218 Sigma

Monoclonal Anti-Cdk4 antibody produced in mouse

clone DCS-31, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, human, rat
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:1,000 using a cultured human tumor cell line extract
clone   DCS-31, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 33 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human Cdk4 protein

General description

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.

Specificity

The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-Cdk4 antibody was used in:
• immunoprecipitation of human melanoma cell line.
• immunofluorescence of fibrosarcoma cells.
• western blot analysis of Notch1 protein. The antibody is also suitable for microarray and western blot at a dilution of 1:1,000 by using a cultured human tumor cell line extract.

Biochem/physiol Actions

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

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Safety & Documentation

Safety Information

WGK Germany  3
Protocols & Articles

Articles

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Peer-Reviewed Papers
15

References

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