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C8218 Sigma

Monoclonal Anti-Cdk4 antibody produced in mouse

clone DCS-31, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, human, rat
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:1,000 using a cultured human tumor cell line extract
clone   DCS-31, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 33 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human Cdk4 protein

General description

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.

Specificity

The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.

Application

Monoclonal Anti-Cdk4 antibody was used in:
• immunoprecipitation of human melanoma cell line.
• immunofluorescence of fibrosarcoma cells.
• western blot analysis of Notch1 protein. The antibody is also suitable for microarray and western blot at a dilution of 1:1,000 by using a cultured human tumor cell line extract.

Biochem/physiol Actions

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

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