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C8232 Sigma

Caspase Combo II, Type 2, 3, and 7 human

>90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym: Caspase Group II

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Properties

Related Categories Apoptosis Enzymes, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, Caspases,
recombinant   expressed in E. coli
assay   >90% (SDS-PAGE)
form   lyophilized powder
solubility   PBS: soluble
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CASP2(835), CASP3(836), CASP7(840)

Description

Biochem/physiol Actions

Group II enzymes function in apoptosis as effectors and upstream activators and used in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

Unit Definition

One unit will hydrolyze 1 nmol of the caspase substrate VDVAD-pNA (for caspase-2) and DEVD-pNA (for caspase-3,7), respectively, per hour at pH 7.2 at 37 °C.

Physical form

Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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