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C8247 Sigma

Anti-CHN1 (6-20) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ARHGAP2, Anti-CHN, Anti-Chimerin (chimaerin) 1, Anti-RHOGAP2

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~53 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CHN1(1123)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 6-20 of human CHN1

General description

CHN1 is a 38-kDa GTPase-activating protein (GAP) and phorbol ester receptor domain. α-chimaerin gene have two isoforms, α1- and α2-chimaerin. Both the isoforms contain C1 and Rho-GAP domains but α2 isoform contains an N-terminal SH2 domain which is absent in α1. It has N-terminal SH2 domain, the C-terminal RhoGAP domain and the central C1 domain similar to protein kinase C.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-CHN1 antibody is suitable for western blot at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

α1-chimaerin is expressed in developing neurons and is controlled by synaptic activity. It plays a crucial role in human neuronal signal-transduction mechanisms. Any alteration in α1-chimaerin expression leads to the pruning of dendritic spines and branches and supression of its expression leads to increased process growth from the dendritic shaft and from spine heads.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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