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C8413 Sigma

Chloramphenicol Acetyltransferase from Escherichia coli

buffered aqueous glycerol solution

Synonym: Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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Properties

Related Categories 2.3.x.x Acyltransferases, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
form   buffered aqueous glycerol solution
activity   50,000-150,000 units/mg protein
mol wt   mol wt 75 kDa (three identical subunits)
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli ... cat(2847485)

Description

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Unit Definition

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Physical form

Clear, colorless solution in 50% glycerol containing 5 mM Tris-HCl, pH 7.8, and 0.5 mM 2-mercaptoethanol

Application

Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.

The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer.

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Amplified Detection

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To standardize a procedure for the determination of the enzymatic assay of Chloramphenicol Acetyltransferase.
Keywords: Enzyme activity, Enzymology, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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