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C8464 Sigma

Monoclonal Anti-CD22 antibody produced in mouse

clone HIB22, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD22

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
  immunohistochemistry (frozen sections): suitable
clone   HIB22, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 135 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD22(933)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

hairy cell leukemia cells.

Antigen Background

CD22 belongs to the immunoglobulin superfamily. It is expressed on the surface of mature B cells and in the cytoplasm of virtually all B cells but not plasma cells. CD22 is strongly expressed in hairy cell leukemia cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD22, 135 kDa transmembrane phosphoglycoprotein. The antibody may be used for detection of both membrane and cytoplasmic CD22 and is suitable for flow cytometry and for staining of acetone-fixed, frozen tissue sections. It binds to an epitope dependent on the presence of both the first and the second Ig-like domains of the CD22 molecule. It does not inhibit CD22 mediated adhesion of various cells to CD22 transfected lines.
5th Workshop: Code No. CD22.14.

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Safety Information

WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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