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C8482 Sigma

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym: Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human



Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in E. coli
assay   >90% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   >1000 units/mg protein
mol wt   mol wt 43.5 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKACA(5566)


Analysis Note

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Biochem/physiol Actions

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

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NONH for all modes of transport
WGK Germany 
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