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C8511 Sigma

Cathepsin C from bovine spleen

Type X, lyophilized powder, ≥5 units/mg protein

Synonym: Dipeptidyl aminopeptidase, Dipeptidyl peptidase I

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Description

Caution

Unstable. Keep frozen.

Packaging

10, 25, 100 units in glass bottle

Unit Definition

One unit will produce 1 μmole of Gly-Phe-NHOH from Gly-Phe-NH2 and hydroxylamine per min at pH 6.8 at 37 °C using DL-phenylalanine hydroxamic acid as the standard. In addition to its hydrolytic properties, cathepsin C catalyzes the polymerization of dipeptide amides.

Physical form

Lyophilized from a 1 M sodium chloride solution.

Application

Cathepsin C has been used in a study that demonstrated the potential of a proteomics approach to identify novel proteins expressed by extravillous trophoblast and to uncover the mechanisms leading to disease states in pregnancy. Cathepsin C has also been used in a study to evaluate biodegradable thermogels.

The enzyme from Sigma has been used in the activation of granzyme k (Gzmk) precursor from E. coli. Granzymes are granule-stored lymphocyte serine proteases that are implicated in T- and natural killer cell-mediated cytotoxic defense reactions.

Biochem/physiol Actions

Cathespin C is a dipeptidyl aminopeptidase that can sequentially remove dipeptides from a peptide chain with an unsubstituted N-terminus. The enzyme exhibits a preference for glycine and proline as N-terminal aminoacids. Substrates that have an N-terminal lysyl or arginyl residue, or a penultimate proryl residue are not targeted by this enzyme. The endopeptidase activity requires the presence of halide ions and sulfydryl activators.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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G1013 Gly-Arg 4-methoxy-β-naphthylamide dihydrochloride, dipeptidyl aminopeptidase I substrate
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S5628 Ser-Tyr β-naphthylamide, powder
P7521 Pro-Arg 4-methoxy-β-naphthylamide acetate salt, powder
G0142 Gly-Phe p-nitroanilide, cathepsin c substrate
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