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C8526 Sigma

Chymopapain from papaya latex

lyophilized powder, ≥2.0 units/mg protein

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Chymopapain,
form   lyophilized powder
composition   Protein, ≥75% E1%/280
solubility   H2O: soluble5.0 mg/mL, clear, colorless
storage temp.   −20°C

Description

Biochem/physiol Actions

Plant cysteine proteinase that is similar in function to lysosomal cathepsin L.

The enzyme cleaves amide linkages at the carboxyl side of arginine, leucine, glutamic acid, and alanine residues; as well as that of arginine esters. The optimum pH varies depending on the kind of substrate. For example, the pH optimum for casein digestion is 7.9, hemoglobin digestion is 4.0 and urea-denatured hemoglobin digestion is 7.0. Molecular mass of the enzyme is found to be 27 KDa.

Other Notes

Contains L-cysteine and EDTA

Preparation Note

Chromatographically purified

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE to Nα-benzoyl-L-arginine per min at pH 6.2 at 25 °C

Application

Chymopapain from papaya latex has been used in a study to assess the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides. Chymopapain from papaya latex has also been used in a study to investigate the preparation and identification of the rabbit′s antibody of chymopapain.

The enzyme has been used to detach cultured CD34+ cells from beads (130 units/mL for 2 minutes, repeated three times). Chymopapain and pronase have been found to be effective in the digestion of human intestinal tissue. The enzyme has been used along with pronase, collagenase, elastase, DNAse, and catalase for rabbit lung cells digestion at a concentration of 0.05% in calcium-magnesium-free Kreb′s serum substitute. It has also been used for the release of neuroblastoma cells from marrow bound to antibody-coated microspheres.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
GC2800000
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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