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  • C8534 - Anti-Chorionic Gonadotropin (α+β subunits) (HCG) antibody produced in rabbit

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C8534 Sigma

Anti-Chorionic Gonadotropin (α+β subunits) (HCG) antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Hormones, Antibodies to Human Plasma Proteins,
species reactivity   human
application(s)   immunohistochemistry: 1:100 using human placental tissue
  radioimmunoassay: 1:50,000
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
storage temp.   −20°C
Gene Information   human ... CGA(1081), CGB(1082)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

HCG

General description

Human Chorionic Gonadotropin (HCG) is a hormone detected in the blood and urine during pregnancy. The peak levels of HCG are detected around Week 12 of gestation and diminish during remaining period of pregnancy. HCG is composed of α and β subunits. There are five variants of HCG that have been identified; HCG, hyperglycosylated HCG, sulphated HCG, free β subunit and pituitary HCG. The important functions of HCG include implantation, angiogenesis, progesterone production, regulation of immune cells and cytotrophoblast differentiation and development of placenta
Anti-Chorionic Gonadotropin (α + α subunits) antibody is specific for both α and β subunits of human chorionic gonadotropin.

Application

Anti-Chorionic Gonadotropin (α + α subunits) antibody may be used for RIA at a minimum working dilution of 1:50,000. For immunohistochemistry, the recommended working dilution is 1:100-1:200. The antibody was also used for immunofluorescence at a working dilution of 1:50 and for ELISA.

Reconstitution

Dilute with buffer: 0.01 M phosphate, pH 7.8, 0.15 M sodium chloride, 0.5% bovine serum albumin and 0.1 sodium azide.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
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