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C8541 Sigma

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse

clone CY-90, ascites fluid

Synonym: Monoclonal Anti-Cytokeratin Peptide 18

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   wide range
application(s)   indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue
  western blot: suitable
clone   CY-90, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

General description

Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins.

Mouse monoclonal clone CY-90 anti-Cytokeratin Peptide 18 antibody shows specific reactivity for the cytokeratin peptide 18 by immunoblotting analysis of human cultured cell lines as well as of human tissue extracts. The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Mouse monoclonal clone CY-90 anti-Cytokeratin Peptide 18 antibody is a homogenous population of antibody molecules that may be used for immunohistochemical staining of formalin fixed, paraffin-embedded or frozen tissue sections by means of indirect immunofluorescence or immunoperoxidase techniques.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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