|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents,|
|mol wt||mol wt ~45 kDa|
|Gene Information||human ... CTSD(1509)|
Contains α, β and γ isoenzymes as observed by isoelectric-focusing.
One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).
Powder containing sodium phosphate buffer salt.
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer’s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
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lyophilized powder, ≥10 units/mg protein
buffered aqueous solution, ≥2,000 units/mg protein (E
sufficient for 120 assays (100 μL)
lyophilized powder, ≥5.0 units/mg protein
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Similarities and differences in the biogenesis, processing and lysosomal targeting between zebrafish and human pro-Cathepsin D: functional implications. Follo C, Ozzano M, Montalenti C, et al. Int. J. Biochem. Cell Biol. 45(2), 273-82, (2013)
A periodate-cleavable linker for functional proteomics under slightly acidic conditions: application for the analysis of intracellular aspartic proteases. Maurer A, Zeyher C, Amin B, et al. J. Proteome Res. 12(1), 199-207, (2013)
4-tert-Octylphenol stimulates the expression of cathepsins in human breast cancer cells and xenografted breast tumors of a mouse model via an estrogen receptor-mediated signaling pathway. Lee HR and Choi KC Toxicology 304, 13-20, (2013)
The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p). Pereira H, Azevedo F, Rego A, et al. FEBS Lett. 587(2), 200-5, (2013)
Role of cathepsin D in U18666A-induced neuronal cell death: potential implication in Niemann-Pick type C disease pathogenesis. Amritraj A, Wang Y, Revett TJ, et al. J. Biol. Chem. 288(5), 3136-52, (2013)
Inhibition of the de-myelinating properties of Aicardi-Goutières syndrome lymphocytes by cathepsin D silencing. Pulliero A, Marengo B, Longobardi M, et al. Biochem. Biophys. Res. Commun. 430(3), 957-62, (2013)
Acidic nanoparticles are trafficked to lysosomes and restore an acidic lysosomal pH and degradative function to compromised ARPE-19 cells. Baltazar GC, Guha S, Lu W, et al. PLoS ONE 7(12), e49635, (2012)
[Influence of isoniazid complex with A-I apolipoprotein on activity of lysosomal enzymes in mice with tuberculous inflammation model]. Sumenkova DV, Poliakov LM, and Panin LE Eksp. Klin. Farmakol. 75(11), 28-30, (2012)
[The role of nicotine as a modulator of the activity of acid phosphotase and cathepsin D and L in the liver and kidney of mice]. Moździerz A, Rzepecka-Stojko A, Juszko-Piekut M, et al. Prz. Lek. 69(10), 833-6, (2012)
ATP13A2 deficiency induces a decrease in cathepsin D activity, fingerprint-like inclusion body formation, and selective degeneration of dopaminergic neurons. Matsui H, Sato F, Sato S, et al. FEBS Lett. 587(9), 1316-25, (2013)
Lysosomal release of cathepsin D precedes relocation of cytochrome c and loss of mitochondrial transmembrane potential during apoptosis induced by oxidative stress. Roberg, K., et al. Free Radic. Biol. Med. 27, 1228-1237, (1999)
An ultrastructural and immunohistochemical study of PC12 cells during apoptosis induced by serum deprivation with special reference to autophagy and lysosomal cathepsins. Ohsawa, Y., et al. Arch. Histol. Cytol. 61, 395-403, (1998)
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