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C8726 Sigma

Caspase 9 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

Synonym: ICE-Lap6, Mch6

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Properties

Related Categories Apoptosis Enzymes, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, Caspase 9,
recombinant   expressed in E. coli (C-terminal histidine-tagged)
assay   ≥90% (SDS-PAGE)
form   buffered aqueous solution
mol wt   N-terminal prodomain plus the large subunit. mol wt 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein)
  small subunit mol wt 13 kDa (subunit containing the histidine tag)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CASP9(842)

Description

Packaging

25 μg in glass bottle

Unit Definition

One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.

Physical form

Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.

Biochem/physiol Actions

When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Parkinson's Disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurod...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 16–20
Keywords: Alzheimer Disease, Apoptosis, Chromatin immunoprecipitation, Clinical, Degradations, Dopamine agents, Gene expression, Parkinson Disease, Polymorphisms

Peer-Reviewed Papers
15

References

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