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C8946 Sigma

α-Chymotrypsin from human pancreas

lyophilized powder

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Chymotrypsin, alpha-,
form   lyophilized powder
mol wt   mol wt 25 kDa
packaging   vial of ≥10 BTEE units
storage temp.   −20°C
Gene Information   human ... CTRB1(1504)

Description

Analysis Note

Protein determined by A1%/280

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25°C.

Application

Human α-chymotrypsin has been used in a study to assess the quantitative structure-activity relationships for organophosphates binding to trypsin and chymotrypsin. Human α-chymotrypsin has also been used in a study to investigate the direct detection of native proteins in biological matrices using extractive electrospray ionization mass spectrometry.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
GC3050000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Procedure for Enzymatic Assay of Chymotrypsin (α‑Chymotrypsin, EC 3.4.21.1)

This procedure is for products with a specification for Chymotrypsin activity. It is not to be used to assay Insoluble Chymotrypsin such as Catalog No. C9134. The procedure is a continuous spectropho...
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
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substrate

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A3401 Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate
S7388 N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide
M4507 4-Methylumbelliferyl p-trimethylammoniocinnamate chloride, powder
S8758 N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate
A9273 Ala-Ala-Val-Ala p-nitroanilide, protease substrate
S1899 N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate
S9761 N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, Leucine aminopeptidase substrate
G3769 Glutaryl-Ala-Ala-Phe-4-methoxy-β-naphthylamide, protease substrate

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