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C8977 Sigma

Monoclonal Anti-CREB antibody produced in rabbit

clone 48H2, purified immunoglobulin, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human, rat, mouse, monkey
application(s)   ChIP: 1:50
  flow cytometry: 1:400
  immunocytochemistry: 1:800
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:6,400
  immunohistochemistry (frozen sections): 1:6,400
  immunoprecipitation: 1:250
  indirect immunofluorescence: 1:800
  western blot: 1:1,000 using extracts from SK-N-MC cells treated with forskolin or FGF
clone   48H2, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous glycerol solution
isotype   IgG
mol wt   antigen mol wt 43 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CREB1(1385)
mouse ... Creb1(12912)
rat ... Creb1(81646)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Recombinant protein specific to amino terminus of human CREB.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin, and < 0.02% sodium azide with 50% glycerol.

Specificity

Detects endogenous levels of total CREB protein.

Legal Information

Rabbit monoclonals produced using Epitomics® Technology, U.S.Patent No. 5,675,063.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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