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C9098 Sigma

EX-CELL® ACF CHO Medium

Animal-component free, without L-glutamine, use at 21.5 g/L, dry powder, suitable for cell culture

Synonym: CHO Medium

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Properties

Related Categories CHO Media and Medium Optimization Kits, CHO Platform, Cell Culture, Serum-free Media
Quality Level   GMP
description   for research or for further manufacturing use
form   dry powder
quality   Drug/Device Master File available
concentration   21.5 g/L
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity.

Other Notes

Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Reconstitution

Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form.

1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Effects of Inhibiting Two Cell Cycle Modulating micrornas

Upper panels: Cells were plated 24 hours after LNA electroporation for the in situ IgG Secretion Assay4. Detection was performed 48 hrs after electroporation. High IgG secretion cutoff is 90th percen...
Nan Lin1, Ken Heuermann,2, Jessica Schlueter,2, Carol Kreader, Scott Knight,2 and Kevin Kayser1
1. Cell Engineering, SAFC, Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103, U.S.A. 2. Research Biotechnology, Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103, U.S.A.
Keywords: Gene expression, Transduction

HTST Treatment

In pharmaceutical manufacturing, viral contamination can pose a significant risk to patient safety and damage a company’s reputation. Processes that reduce the risk of viral contamination, such as Hi...
Aaron Mack, Daiva Dailide, Chas Hernandez, Erik Klahn, and Bruce Lehr
Sigma-Aldrich Corporation, Cell Sciences and Development, 2909 Laclede Ave., St. Louis, MO, 63103
Keywords: Amino acid analysis, Cell culture, Culture media, Degradations, Elemental analysis, High performance liquid chromatography, Inductively coupled plasma, Infrared spectroscopy, Mass spectrometry, Pharmaceutical, Precipitation, Reductions, Spectroscopy, Titrations, Vitamins

Identification of the Putative Rosa26 Locus

The biopharmaceutical industry has expressed considerable interest in targeted integration in CHO cells for therapeutic r-protein production applications. In previous studies we have used ZFN’s for s...
Scott Bahr, Nan Lin, Trissa Borgschulte, Jeanne Brooks, Henry George and Kevin Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich, St Louis, MO 63103 USA
Keywords: Cloning, Gene expression, Polymerase chain reaction, Sequencing

Improving Product Safety Profiles

Post-translational modifications have been shown to affect the bioactivity, clearance rates, immunogenicity and safety profiles of therapeutic glycoproteins. For example anti- N-glycolylneuraminic ac...
Mascarenhas, J., Achtien, K., Richardson, S., Sealover, N., Kaiser, J., Borgschulte, T., George, H., Kayser, K. and Lin, N
Cell Sciences and Development, SAFC Sigma Aldrich2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Cell culture, Clinical, Eliminations, Gene expression, Glycosylations, High performance liquid chromatography, Mass spectrometry, Sequencing, Size-exclusion chromatography

MSX Amplification

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992). However, most Chinese Hamster Ovary (CHO) c...
Kate Achtien, Trissa Borgschulte, Nan Lin, Henry George, Kevin J. Kayser
Cell Sciences and Development, SAFC, Sigma-Aldrich, 2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Amplification, Cloning, Gene expression, Indicators, Polymerase chain reaction, Polymerase chain reaction - quantitative

Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1...
Nan Lin, Dustin Davis, Natalie R. Sealover, Joaquina Mascarenhas, Henry J. George and Kevin J. Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich 2909 Laclede Ave., Saint Louis, MO 63103. U.S.A.
Keywords: Affinity chromatography, Chromatography, Gene expression, Polymerase chain reaction, Protein extraction

Overexpression of Serpinb1

We report the discovery and validation of a novel CHO cell engineering target (Serpinb1) that has significant effects in enhancing recombinant IgG productivity. We performed transcriptomic studies us...
Nan Lin, Jeanne Brooks, Natalie Sealover, Christopher Limmex, Henry J. George and Kevin J. Kayser
Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103 USA
Keywords: Gene expression, High performance liquid chromatography, Inflammation, Microarray Analysis, Transcription, Transduction, Transfection, cDNA microarrays

Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identified species-specific housekeeping genes (HKGs) for Chinese HamsterOvary (CHO) cells using data from microarray gene expression profiling. HKGs suitable for quanti...
Scott M. Bahr, Trissa Borgschulte, Kevin J. Kayser, Matthew V. Caple and Nan Lin
Cell Sciences and Development, SAFC Biosciences 2909 Laclede Avenue, Saint Louis, MO 63103, USA
Keywords: Amplification, Cell culture, DNA microarrays, Gas chromatography, Gene expression, Melting, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction, Transcription

Signal Peptide Optimization

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins. Signal peptides are known to have a strong impact on both the efficiency of protein secretion and c...
Joaquina Mascarenhas, Dustin Davis, Pegah Jalili, Trissa Borgschulte, Kevin Ray, Henry J. George, Kevin J. Kayser and Nan Lin
Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Ave., Saint Louis, MO 63103. U.S.A.
Keywords: Atomic absorption spectroscopy, Cell culture, Gene expression, High performance liquid chromatography, Mass spectrometry, Size-exclusion chromatography, Transfection

Zinc Finger Nuclease Efficiency

Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. Using current methods, the efficien...
Scott Bahr, Laura Cortner, Sara Ladley, Trissa Borgschulte, CHOZN® Platform Development Team
SAFC/Sigma-Aldrich, St Louis, MO 63103 USA, scott.bahr@sial.com
Keywords: Gene expression, Recombination, Sequencing

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