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  • C9102 - Anti-phospho-CREB (pSer133) antibody produced in rabbit

C9102 Sigma

Anti-phospho-CREB (pSer133) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, human, rat
application(s)   immunoprecipitation: 1:50
  western blot: 1:1,000 using extract from SK-N-MC cells, untreated or forskolin treated.
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 43 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CREB1(1385)
mouse ... Creb1(12912)
rat ... Creb1(81646)
biological source   rabbit
conjugate   unconjugated



synthetic phosphopeptide corresponding to the region of human CREB that contains serine 133.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin, and 50% glycerol.


The antibody detects CREB when phosphorylated at Ser133 and phosphorylated ATF-1.


Anti-phospho-CREB (pSer133) antibody produced in rabbit may be used for immunoprecipitation at a working dilution of 1:50 and for immunoblotting at a working dilution of 1:1000 using an extract from SK-N-MC cells, untreated or forskolin treated. It was used for western blot analysis of proteins extracted from vascular smooth muscle cell cultures, separated by SDS-PAGE and blotted onto nitrocellulose membranes in a study. It was used to assess CREB activation by western blotting in a study.

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Biochem/physiol Actions

CREB is a phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Various extracellular signals including cAMP, membrane depolarization, increased intracellular Ca2+, and growth and neurotrophic factors stimulate this protein to activate transcription. Phosphorylation of CREB takes place at Ser133 and this regulates the ability of the protein to activate transcription. Studies show that CREB is a mediator in learning and long-term memory in both flies and mice.

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WGK Germany  2
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