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C9108 Sigma

Monoclonal Anti-Chk2 antibody produced in mouse

~2 mg/mL, clone DCS-273, purified immunoglobulin, buffered aqueous solution


Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  microarray: suitable
  western blot: 2-4 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
clone   DCS-273, monoclonal
concentration   ~2 mg/mL
  ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 61 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CHEK2(11200)
biological source   mouse
conjugate   unconjugated



recombinant human Chk2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.


The epitope recognized by the antibody resides within the FHA domain of the Chk2 molecule. This epitope is likely to be masked by protein-protein interactions in vivo.


Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) and for immunoblotting at a concentration of 2 to 4 μg/mL using whole extract of cultured 293T cells.

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Biochem/physiol Actions

The protein propagates signals from damaged or unreplicated DNA. The protein is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.

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