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C9142 Sigma

Cyanogen bromide-activated-Sepharose® 4B

lyophilized powder

Synonym: Cyanogen bromide-activated Agarose

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Properties

Related Categories Activated / Functionalized Matrices, Activated/Functionalized Matrices, Affinity Chromatography, Molecular Biology, Protein Chromatography,
form   lyophilized powder
matrix   Sepharose 4B
matrix active group   cyanate or related structures
matrix spacer   1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling   1 g swells to 4-5 mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Cyanogen bromide-activated-Sepharose® is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose® has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How are proteins attached to the Cyanogen bromide-activated Sepharose® in Product C9142?
Coupling of molecules to CNBr-activated Sepharose® is usually via amine groups.  In the case of proteins, this usually means the epsilon amines (exocyclic amines) of lysine groups.
After I've washed and prepared the resin in Product C9142, Cyanogen bromide-activated-Sepharose®, for the coupling reaction, how long can I wait before coupling the ligand to the resin?
We would recommend coupling the ligand immediately after washing and preparing the resin.  This is because the reactive groups hydrolyze in the basic solution conditions of the preparation steps.  The solutions used in the coupling reaction should also be kept cold (2-8°C) during the procedure.
What kind of volume should I use for my ligand to couple to the Cyanogen bromide-activated Sepharose®, Product C9142?
It is best that the coupling ligand should not be in too dilute a solution.  In general, a volume ratio of 0.5 to 1 of coupling solution to CNBr-activated Sepharose® is recommended.
My ligand is in Tris buffer.  Can I use Product C9142, Cyanogen bromide-activated-Sepharose®, with this sample?
Because Tris is an amine-containing buffer, we do not recommend coupling of samples in Tris buffer to the CNBr-activated Sepharose®.  Tris will react with the CNBr groups and remove sites for ligand coupling.  We would not recommend use of any amine-containing buffer in the coupling reaction.  However, after the ligand has been added in the coupling reaction, then it is OK to use amine-containing buffers to block the remaining sites (e.g. ethanolamine, glycine).
When using Product C9142, Cyanogen bromide-activated-Sepharose®, is there a particular storage buffer that I should use after I've coupled my ligand to the resin?
This depends on the particular situation, and the answer depends more on the coupled ligand than the resin itself.  The most important criterion is to choose a buffer that is not harmful to the ligand.  It is common to include 20% ethanol or preservatives like sodium azide or thimerosal (~0.02%) to inhibit potential bacterial growth.
Should I use a desiccator environment for storage of Cyanogen bromide-activated-Sepharose®?
It is a good idea to store the CNBr-activated agarose products in as dry an environment as possible, because the reactive groups are very moisture-sensitive.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
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References

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C9210 Cyanogen bromide-activated Agarose, lyophilized powder
08168 Timestrip Plus 8 °C

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