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  • C9179 - Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse

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C9179 Sigma

Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse

clone CK-E3, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   human, bovine, goat, rat, pig
application(s)   indirect immunofluorescence: 1:400 using acetone-fixed frozen sections of human salivary gland
  microarray: suitable
  western blot: suitable
clone   CK-E3, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... KRT17(3872)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytoskeletal preparation from rat colon epithelium.

General description

Cytokeratin 17, with a molecular weight of 46 kDa, is a member of the type I acidic subfamily of cytokeratins that is expressed in normal human epithelial tissues such as glandular epithelium with myoepithelial component, transitional and pseudostratified epithelia. This protein may be involved in the generation of spatial organization of epithelial tissues.

Specificity

Recognizes specifically human cytokeratin 17 (46-47 kDa) and rat cytokeratin 19 (40 kDa). The antibody labels intermediate filaments in human cultured epitheloid carcinoma cell line (HeLa) and in rat hepatoma cells, but does not stain rat fibroblasts in primary cultures. It stains a minor basal cell population in all variants of complex epithelia; strong staining was noted in myoepithelial cells of all glands studied. The antibody exhibits a wide interspecies cross-reactivity, however, the recognized cytokeratin was not studied in cases other than human and rat. The epitope detected by the antibody is sensitive to formalin fixation.

Application

Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse is suitable for western blotting, microarray and indirect immunofluorescence at a working dilution of 1:400 using acetone-fixed frozen sections of human salivary gland. It recognizes an epitope present in epithelial tissues and may be used for typing of normal, metaplastic and neoplastic cells. It may be useful in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It may also be used for the localization of cytokeratin 17 using various immunochemical assays such as dot blotting and immunohistochemistry. It has been used in the detection of expression of inducible keratin k17 in human keratinocytes in pachyonychia congenita patients.

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