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C9233 Sigma

Anti-Chk2 antibody, Mouse monoclonal

clone DCS-270, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Chk2 antibody produced in mouse

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation1: suitable
  microarray: suitable
  western blot1: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
clone   DCS-270, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 61 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CHEK2(11200)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human Chk2.

General description

The protein encoded by this gene is a serine/threonine-protein kinase that acts as a cell cycle checkpoint regulator and a putative tumor suppressor. It contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA), which is activated by rapid phosphorylation in response to replication blocks and DNA damage.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The epitope recognized by the antibody resides within the N-terminal regulatory domain of the Chk2 molecule.

Application

Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunocytochemistry, immunohistochemistry (formalin-fixed, paraffin-embedded sections), microarray, immunoprecipitation and for immunoblotting at a concentration of 1-2 μg/mL using whole extract of cultured 293T cells. It was used for Chk2 immunoprecipitation experiments to study the engagement of the ATR-dependent DNA damage response at the human papillomavirus 18 replication centers during the initial amplification

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Biochem/physiol Actions

The protein propagates signals from damaged or unreplicated DNA. It is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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