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C9284 Sigma

Catalase−Agarose

pH 5.4(isoelectric point), ammonium sulfate suspension, catalase from bovine liver

Synonym: H2O2:H2O2 Oxidoreductase

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Properties

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
biological source   catalase from bovine liver
form   ammonium sulfate suspension
mol wt   tetramer mol wt ~250 kDa
extent of labeling   60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)
optimum pH   ~7.0
matrix   4% cross-linked beaded agarose
pH   5.4(isoelectric point)
storage temp.   2-8°C

Description

Caution

Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 7, containing 0.02% thimerosal

Application

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.

Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H2O2) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H2O2) that can degrade the target product.

Biochem/physiol Actions

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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A8056 3-Amino-1,2,4-triazole, ≥95% (TLC)

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