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C9336 Sigma

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Miscellaneous Antibodies, Miscellaneous Antibodies for Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
mol wt   antigen mol wt 26 kDa
application(s)   indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
  western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

bacterial chloramphenicol acetyl transferase (CAT).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Chloramphenicol Acetyl Transferase (CAT) is suitable for use in immunoblotting and indirect immunofluorescence (10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

General description

Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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