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C9454 Sigma

Citrate Synthase from Thermoplasma acidophilum

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥5 units/mg protein (biuret)

Synonym: Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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Properties

Related Categories 4.1.x.x C-C bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
recombinant   expressed in E. coli
form   ammonium sulfate suspension
foreign activity   aconitase, essentially free
  isocitrate dehydrogenase <0.05%
  malic dehydrogenase <0.1%
storage temp.   2-8°C

Description

Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0, containing 50 mM phosphate

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Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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