C9584 Sigma

Carboxypeptidase B from porcine pancreas

lyophilized powder

DOWNLOAD MSDS (PDF)

Synonym: Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase

Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   lyophilized powder
activity   ≥125 units/mg protein
purified by   affinity chromatography
composition   Protein, 40-70%
foreign activity   carboxypeptidase A ≤1%
  chymotrypsin ≤0.1%
  trypsin ≤2%
storage temp.   −20°C

Description

Analysis Note

Protein determined by biuret.

Packaging

Package size based on protein content

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Physical form

Contains HEPES buffer salts and carbohydrate

Application

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

Price and Availability

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