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  • C9602 - Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit

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C9602 Sigma

Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chloride Channels, Antibodies to Ion Channels,
species reactivity   rat
application(s)   western blot (chemiluminescent): 1:200-1:300 using rat heart membranes and ECL.
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   rat ... Clcn3(84360)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

highly purified GST fusion protein of the rat chloride channel-3 (CLC-3) corresponding to amino acids 592-661.

General description

The protein contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function.

Physical form

Lyophilized at ~0.3 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose, and 0.025% sodium azide.

Specificity

The epitope has significant homology with CLC-4 and CLC-5 proteins in rat and is well conserved in vertabrates.

Application

Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit is suitable for western blotting (chemiluminescent) at a working dilution of 1:200-1:300 using rat heart membranes and ECL. It was used as a primary antibody for western blotting at a dilution of 1:2000 to demonstrate adenoviral-mediated overexpression of ClC-3.

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

The encoded protein, voltage-gated chloride channel, is a transmembrane protein that maintains chloride ion homeostasis in various cells. It contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. ClC-3 deficiency inhibits Ang II-induced apoptosis of endothelial progenitor cells via suppressing ROS (reactive oxygen species) generation derived from NADPH oxidase. Osmotically active Cl- is extruded via this channel upon kinase activation, which facilitates cytoplasmic condensation preceding cell division. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma.

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