Synonym: γ-Glutamyl hydrolase
|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carboxypeptidase G,|
|composition||Protein, ~70% biuret|
One unit will hydrolyze 1.0 μmole of
Contains sodium acetate salt.
Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.
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(Type II-PMSF treated), ≥50 units/mg protein, aqueous suspension
≥50 units/mg protein
lyophilized powder, ≥50 units/mg protein
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Genetic Polymorphism of γ-Glutamyl Hydrolase in Chinese Acute Leukemia Children and Identification of a Novel Double Nonsynonymous Mutation. Chen, X., et al. Pediatr. Hematol. Oncol. 29, 303-12, (2012)
Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate. potent inhibitors of glutamate carboxypeptidase II. Tsukamoto T, Flanary JM, Rojas C, et al. Bioorg. Med. Chem. Lett. 12(16), 2189-92, (2002)
Baculoviral expression and characterization of human recombinant PGCP in the form of an active mature dimer and an inactive precursor protein. Zajc T, Suban D, Rajković J, et al. Protein Expr. Purif. 75(2), 119-26, (2011)
Cathepsin C and plasma glutamate carboxypeptidase secreted from Fischer rat thyroid cells liberate thyroxin from the N-terminus of thyroglobulin. Suban D, Zajc T, Renko M, et al. Biochimie 94(3), 719-26, (2012)
TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus. Suzaki T, Kim CS, Takeda N, et al. Development 140(2), 353-61, (2013)
Carboxypeptidase displaying differential velocity in hydrolysis of methotrexate, 5-methyltetrahydrofolic acid, and leucovorin. Albrecht AM, Boldizsar E, and Hutchison DJ J. Bacteriol. 134(2), 506-13, (1978)
Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS-16. Use of a novel triazine dye affinity method. Sherwood RF, Melton RG, Alwan SM, et al. Eur. J. Biochem. 148(3), 447-53, (1985)
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