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C9672 Sigma

Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse

clone NCAM-0B11, ascites fluid

Synonym: Anti-CD56-CY, Anti-NCAM



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human, rat
application(s)   immunocytochemistry: suitable using cells and tissues
  western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract
clone   NCAM-0B11, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 140-180 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NCAM1(4684), NCAM2(4685)
rat ... Ncam1(24586), Ncam2(288280)
biological source   mouse
conjugate   unconjugated



growth cone enriched plasma membrane fraction from E17 rat forebrain.

General description

Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot.

Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain as a family of sialoglycoproteins of molecular weights 180 kDa, 140 kDa and 120 kDa, which arise from alternative splicing of mRNA transcribed from a single gene. N-CAMs are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (3 papers)

Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody is used to tag neural cell adhesion molecule for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting. It is used as a probe to determine the presence and roles of neural cell adhesion molecule in cell-cell interactions during embryogenesis and development.

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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