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C9687 Sigma

Monoclonal Anti-Cytokeratin, pan (Mixture) Prediluted antibody produced in mouse

clone C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, ascites fluid

Synonym: Monoclonal Anti-Cytokeratin, pan (mixture)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   wide range
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
clone   C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, monoclonal
antibody form   ascites fluid
isotype   IgG1/IgG2a
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

mixture of several monoclonal cytokeratin clones.

General description

Cytokeratins are cytoplasmic structural proteins that are characteristic of epithelial and trichocytic cells. The group contains at least 29 different proteins.

Physical form

Prediluted containing horse serum, phenol red, and 15 mM sodium azide.

Specificity

This is a mixture of monoclonal anti-cytokeratin antibodies. It recognizes human cytokeratins 1,4,5,6,8,10,13,18, and 19. It is a broad spectrum reagent, which reacts specifically with a wide variety of normal, reactive, and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Increased staining intensity is seen following proteolytic treatment (protease unmasking).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-Pan Cytokeratin (mixture) produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoblotting and dot blotting. It is also useful for staining of cultured epithelial cell lines. Staining can be amplified by synergy between the various components and this enables identification of cells which would otherwise be stained only marginally. It aids in the discrimination of carcinomas and non-epithelial tumors like sarcomas, lymphomas, and neural tumors. It detects micrometastases in lymph nodes, bone marrow, and other tissues and may be used for determining the origin of poorly differentiated tumors. The mixture recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic, and neoplastic cells. It was used for immunostaining in a study to demonstrate differential expression and function of cadherin-6 during renal epithelium development. It was used for whole mount immunofluorescence to detect cytokeratin and for visualization of nephric duct derivatives in a study.

Features and Benefits

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Linkage

This product is similar to C2562, but is offered prediluted and ready to use.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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