C9848 Sigma

Monoclonal Anti-Calbindin-D-28K antibody produced in mouse

clone CB-955, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies for Neural Stem Cells,
antibody form   ascites fluid
clone   CB-955, monoclonal
mol wt   antigen mol wt 28 kDa
contains   15 mM sodium azide
species reactivity   goat, sheep, canine, guinea pig, bovine, pig, rat, human, feline, rabbit, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using rat cerebellum sections
  indirect ELISA: suitable
  western blot: 1:3,000 using MDBK cell extract
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)

Description

Features and Benefits

Antibody Bioguarantee
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Immunogen

bovine kidney calbindin-D

Specificity

The antibody does not react with other members of the EF-hand family, such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S-100A2 (S100L) and S-100A6 (calcyclin). A weaker reactivity was observed with chicken calbindin-D-28K.

Application

The antibody may be used in immunohistochemistry (alcohol-, formalin-, and methacarn-fixed, paraffin-embedded, and frozen tissue sections). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections improves immunohistochemical staining.

Price and Availability

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